XGenomes is an early-stage startup in Cambridge, MA building innovative sequencing technologies to unlock the power of genomics for the future of healthcare. We are reinventing sequencing to personalize medicine and accelerate diagnostics. Our mission is to make multi-omic information accessible throughout an individual’s lifetime, detect early signals of disease, and to monitor the effects of interventions. We are looking for a versatile, hands-on Senior Scientist to join our R&D team to help shape and develop our innovative sequencing platform. This role sits at the intersection of single-molecule imaging, molecular biology, and sequencing chemistry, and is well-suited to someone who thrives in an interdisciplinary startup environment where everyone contributes beyond their core specialty, and work-life balance is valued. We look forward to reading your application at this link! What we offer- The opportunity to shape foundational sequencing technology from the ground up- A collaborative, scientifically rigorous, and intellectually curious team- Access to The Engine accelerator community and resources- Salary: $90k – $120k plus equity- PTO: 10 holidays/year, 15+ vacation days/year; 5 sick days/year What you'll do- Design, execute, and interpret sequencing experiments supporting assay development, with an emphasis on single-molecule imaging data- Analyze imaging and sequencing data, draw actionable conclusions, and iterate quickly on protocols- Develop and troubleshoot sequencing chemistry and workflows, from sample prep through data generation- Apply molecular biology skills (cloning, PCR, library prep, nucleic acid handling, enzymatic reactions) to support assay development- Lead key demonstration of XGenomes’ technological capabilities for specific clinical assay applications- Document experimental procedures, results, and protocols clearly for internal knowledge-sharing- Collaborate cross-functionally with engineering and software teams to characterize, calibrate, and improve imaging instrumentation- Take on responsibilities outside your core specialty as needed Required Qualifications- PhD in Biophysics, Chemistry, Biochemistry, Molecular Biology, Bioengineering, or a related field, or a Master's degree with 3+ of equivalent hands-on industry research experience- Demonstrated experience with fluorescence microscopy techniques- Solid foundation in basic molecular biology techniques (PCR, cloning, nucleic acid purification, enzymatic assays)- Strong experimental rigor: able to design controls, troubleshoot independently, and interpret data- Excellent communication skills Preferred / Bonus Experience- Single-molecule localization microscopy (SMLM)- 3D printing for rapid prototyping of lab hardware or instrumentation components- Designing, integrating, or troubleshooting microfluidics or fluidics systems- Designing, building, or troubleshooting optical systems- Chemical synthesis, modification, or handling, particularly of nucleic acids or fluorescent dyes- Developing and using modern sequencing technologies- Liquid biopsy field- Computer programming (Julia, Python, MATLAB, Linux, AWS)- Computer vision and machine learning methods (not LLMs)